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Intra and inter‐user reliability of central corneal thickness measurements obtained in healthy feline eyes using a portable spectral‐domain optical coherence tomography device
Author(s) -
Alario Anthony F.,
Pirie Christopher G.
Publication year - 2013
Publication title -
veterinary ophthalmology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.594
H-Index - 50
eISSN - 1463-5224
pISSN - 1463-5216
DOI - 10.1111/vop.12029
Subject(s) - optical coherence tomography , ophthalmology , significant difference , medicine , coefficient of variation , mean difference , reliability (semiconductor) , corneal pachymetry , cornea , nuclear medicine , mathematics , biomedical engineering , corneal topography , physics , statistics , confidence interval , power (physics) , quantum mechanics
Objective To determine the intra and inter‐user reliability of central corneal thickness ( CCT ) measurements obtained using a portable spectral‐domain optical coherence tomography ( SD ‐ OCT ) device in healthy feline eyes. Animals Twenty clinically normal cats. Procedures Gentle manual restraint was utilized for proper animal positioning. Central corneal thickness measurements were obtained from both eyes of each animal three times by two operators in succession. A 6 mm, corneal pachymetry protocol was performed using the Optovue ® iVue SD ‐ OCT system. The CCT was automatically calculated using the pachymetry software generated from eight radial scans, 6 mm in length. The CCT was recorded for each operator and compared statistically for both intra and inter‐user reliability. Results Twenty cats (11 CM, 9 SF ) with a mean age of 4.7 years were examined. The mean CCT for all eyes examined was 629.08 ± 47.05 μm. No significant difference in CCT was found based on the eye examined ( OD vs. OS ) or age of the animal. However, the CCT of spayed females was significantly higher (42.64 ± 18.0 μm) as compared to castrated males ( P = 0.03). There was no significant difference in CCT measurements between replicates performed by the same operator or between operators. The mean difference in CCT between operators was 0.38 μm. The coefficient of variation for each user and between users was very low (range 0.68–1.5%). The intra‐class correlation coefficient comparing operators was 0.975. Conclusions The Optovue ® iVue SD ‐ OCT device is capable of obtaining CCT measurements with excellent intra and interoperator reliability in healthy feline eyes.