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Variability of tissue factor‐activated thromboelastography and whole blood impedance platelet aggregometry in healthy dogs
Author(s) -
Shropshire Sarah B.,
Olver Christine S.,
Lappin Michael R.
Publication year - 2018
Publication title -
journal of veterinary emergency and critical care
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.886
H-Index - 47
eISSN - 1476-4431
pISSN - 1479-3261
DOI - 10.1111/vec.12733
Subject(s) - thromboelastography , hematocrit , medicine , partial thromboplastin time , whole blood , platelet , fibrinogen , prothrombin time , clotting time , thrombelastography , activated clotting time , platelet poor plasma , anesthesia , cardiology , thrombin , heparin
Objective To assess interindividual (CV G ) and intraindividual (CV I ) variability over time for results of thromboelastography (TEG) and whole‐blood impedance platelet aggregometry in healthy dogs. Animals Six healthy Beagle dogs. Measurements and Main Results Tissue factor (TF)‐activated TEG and adenosine diphosphate (ADP) and arachidonic acid (AA)‐induced whole blood impedance platelet aggregometry were performed at 3 different time points (days 1, 4, and 6). In addition, platelet count, hematocrit, and plasma fibrinogen concentrations were recorded each study day. Activated partial thromboplastin time, one‐stage prothrombin time, antithrombin activity, and D‐dimer concentrations were measured on the first day of the study. For TEG, the variables reaction time ( R ), clotting time ( K ), rate of clot formation ( α ), and maximum amplitude (MA) were recorded. For platelet aggregometry, the areas under the curve for ADP (AUC ADP )‐ and AA (AUC AA )‐induced aggregation were measured. The CV I was lower than the CV G over time for MA, AUC ADP , and AUC AA ; however, the CV I was higher than the CV G for the TEG variables R , K , and α . There were no statistical differences in the platelet count, hematocrit, and fibrinogen measurements over time. Conclusions In healthy dogs, a subject‐based reference interval for ADP‐ and AA‐induced platelet aggregometry and the TEG variable MA provide a more sensitive method to detect changes. However, due to the high CV I , population‐based reference intervals may be more appropriate for interpretation of the TEG variables R , K , and α .