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Serum myoglobin, creatine kinase, and cell‐free DNA in endurance sled dogs and sled dogs with clinical rhabdomyolysis
Author(s) -
Devall Veronica C.,
Goggs Robert,
Hansen Christina,
Frye Christopher W.,
Letendre JoAnnie,
Wakshlag Joseph J.
Publication year - 2018
Publication title -
journal of veterinary emergency and critical care
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.886
H-Index - 47
eISSN - 1476-4431
pISSN - 1479-3261
DOI - 10.1111/vec.12731
Subject(s) - rhabdomyolysis , myoglobin , creatine kinase , medicine , creatine , biomarker , endocrinology , cardiology , biochemistry , biology
Abstract Objective To evaluate whether cell‐free DNA (cfDNA) concentrations are increased in dogs with exertional rhabdomyolysis and whether concentrations are correlated with serum myoglobin concentration and creatine kinase activity. Design Observational cohort study. Setting Yukon Quest 1,000‐mile International Sled Dog Race 2015. Animals Twelve normal competitive sled dogs; 5 dogs with rhabdomyolysis. Interventions None. Measurements and Main Results Blood was collected from all confirmed cases of exertional rhabdomyolysis and compared to the winning team at the midrace point. Results indicate that median cfDNA did not increase, but decreased by the race finish (prerace = 314.2 ng/mL versus midrace = 283.7 ng/mL versus postrace = 249.5 ng/mL). There were no rises in median cfDNA in dogs with rhabdomyolysis (255 ng/mL) negating its potential utility as a measure of acute skeletal muscle compromise. In contrast, myoglobin concentration and creatine kinase activity at the midrace point for normal dogs were significantly lower than dogs with rhabdomyolysis. Values for myoglobin and creatine kinase were strongly positively correlated ( R = 0.91). Conclusions cfDNA is not a useful biomarker for exertional rhabdomyolysis in contrast to myoglobin and creatine kinase. Further evaluation of timing and clinical signs suggests that exertional rhabdomyolysis occurs early in endurance activities. Among the dogs with rhabdomyolysis, the dog that demonstrated clinical signs had the highest serum creatine kinase activity and myoglobin concentration.