Premium
Evaluation of an immunochromatographic test for feline AB system blood typing
Author(s) -
Spada Eva,
Proverbio Daniela,
Baggiani Luciana,
Bagnagatti De Giorgi Giada,
Perego Roberta,
Ferro Elisabetta
Publication year - 2015
Publication title -
journal of veterinary emergency and critical care
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.886
H-Index - 47
eISSN - 1476-4431
pISSN - 1479-3261
DOI - 10.1111/vec.12360
Subject(s) - medicine , blood typing , whole blood , repeatability , mannitol , typing , agglutination (biology) , chromatography , antibody , immunology , microbiology and biotechnology , chemistry , biology , biochemistry
Objective To determine the accuracy of an immunochromatographic cartridge (IC) test for blood typing feline type A, B, and AB blood samples. Design Prospective observational study. Setting University teaching hospital. Samples Fifty‐one nonanemic and 19 anemic feline blood samples. Interventions Samples were blood typed by both card agglutination (CA) and IC techniques. Discordant results were analyzed using a back‐typing technique for the presence of alloantibodies. Repeatability and reproducibility of the IC method were evaluated. Accuracy of the IC method was determined for feline whole blood anticoagulated with EDTA and citrate phosphate dextrose adenine (CPDA1), for feline‐packed RBCs with CPDA1 and saline adenine glucose‐mannitol, and for autoagglutinated and hemolytic samples. Accuracy of IC testing was determined for feline blood after room temperature, 4 ± 2, −20, and −80°C storage following 24, 48, 72, and 96 hours, and after 1, 2, 3, and 4 weeks of storage. Measurements and Main Results In anemic and nonanemic samples the IC technique had a specificity, sensitivity, positive predictive value, and negative predictive value of 100% in detecting feline blood types A, B, and AB, outperforming a CA test. Results were repeatable and reproducible. Using IC it was possible to blood type samples anticoagulated with EDTA and CPDA1, packed RBC samples with CPDA1 and saline adenine glucose‐mannitol, autoagglutinated and hemolytic samples, and samples stored at 4 ± 2°C and at room temperature for up to 1 month. Conclusions The IC technique is an accurate assay for the identification of A, B, and AB blood types in anemic and nonanemic feline blood. It has a higher sensitivity and specificity than the CA test, and can be used in samples stored with common anticoagulants or preservative solutions used in feline transfusion medicine.