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Evaluation of two point‐of‐care ethylene glycol tests for dogs
Author(s) -
Creighton Karina J.,
Koenigshof Amy M.,
Weder Christian D.,
Jutkowitz L. Ari
Publication year - 2014
Publication title -
journal of veterinary emergency and critical care
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.886
H-Index - 47
eISSN - 1476-4431
pISSN - 1479-3261
DOI - 10.1111/vec.12203
Subject(s) - venipuncture , medicine , vial , ethylene glycol , point of care testing , point of care , chromatography , nuclear medicine , surgery , pathology , chemistry , organic chemistry
Objective To evaluate 2 point‐of‐care ethylene glycol (EG) tests in dogs. Design Prospective, randomized, blinded laboratory evaluation. Setting University teaching hospital. Animals Ten healthy adult dogs. Interventions Jugular venipuncture and in vitro evaluation for detection of EG in canine blood. Measurements Whole blood samples were centrifuged and separated, and the plasma was divided into 30 aliquots. The aliquots were mixed with EG to provide EG concentrations ranging from 0 to 100 mg/dL. The EG concentration of each sample was confirmed using gas chromatography. For the VetSpec EG Qualitative Reagent Test Kit, 100 μL of each sample was added to test vials and compared with 20 and 50 mg/dL reference vials. For the Kacey EG Test Strips, 20 μL of each sample was added to the test circle and compared with the color chart provided by the manufacturer. For each test, samples were prepared in groups of 5 and presented in randomized order to 2 readers who were blinded to the presumed EG concentration. Samples were scored as negative, 20–50 mg/dL, or greater than 50 mg/dL. For each test, the sensitivity and specificity for detecting EG was calculated. Cohen's unweighted kappa coefficient was calculated to determine the degree of agreement between readers. Main Results For detecting EG, the Kacey EG Test Strips had excellent sensitivity and specificity (both 100%) and good agreement between readers. The VetSpec EG Qualitative Reagent Test Kit was less sensitive and specific (65% and 70% for the first reader, 95% and 40% for the second) with less agreement. Conclusions Of the 2 systems evaluated, the Kacey EG Test Strips displayed greater accuracy and ease of use.