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Comparison of a gel column blood typing method and a point‐of‐care cartridge for D og E rythrocyte A ntigen 1.1
Author(s) -
Blois Shauna L.,
Richardson Danielle M.,
AbramsOgg Anthony C. G.
Publication year - 2013
Publication title -
journal of veterinary emergency and critical care
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.886
H-Index - 47
eISSN - 1476-4431
pISSN - 1479-3261
DOI - 10.1111/vec.12052
Subject(s) - typing , cartridge , medicine , blood typing , point of care , point of care testing , whole blood , antigen , immunology , pathology , biology , microbiology and biotechnology , mechanical engineering , engineering
Background Blood typing for the presence of D og E rythrocyte A ntigen ( DEA ) 1.1 is recommended in all donor and recipient dogs prior to transfusion of blood products. The objective of this study was to determine if a point‐of‐care DEA 1.1 blood typing cartridge could be used in place of the gel column typing method. Study Design Detection of DEA 1.1 was performed using a laboratory‐based gel column method and a point‐of‐care cartridge. A convenience sample of 30 healthy blood donors, 13 dogs with immune‐mediated hemolytic anemia ( IMHA ) (3 of which had concurrent immune‐mediated thrombocytopenia [ IMT ]), and 44 dogs with other diseases was included in the study. Key Findings Agreement was observed between the tests for normal dogs and dogs with nonimmune‐mediated disease in 74/74 cases. Two dogs in the IMHA group had indeterminate gel column blood typing results; 1 dog in this group had a negative gel column test result but a positive cartridge test result. Significance There was good agreement between the 2 methods for normal dogs and dogs with nonimmune‐mediated disease. Blood typing methods in dogs with IMHA should be further investigated.