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Validation of a diagnostic tool for the diagnosis of lumpy skin disease
Author(s) -
Mwanandota Julius J.,
Macharia Mercy,
Ngeleja Chanasa M.,
Sallu Raphael S.,
Yongolo Mmeta G.,
Mayenga Charles,
Holton Timothy A.
Publication year - 2018
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/vde.12690
Subject(s) - loop mediated isothermal amplification , medicine , virology , biology , dna , genetics
Background Lumpy skin disease ( LSD ) is caused by LSD virus which is a member of the Capripoxvirus (Ca PV ) genus. Although PCR provides for a rapid and sensitive diagnosis, it has limited use due to its complexity in terms of cost, time and equipment. Loop‐mediated isothermal amplification ( LAMP ) is a simple, specific and cost‐effective method with a diagnostic accuracy similar to PCR . Objectives/Hypothesis To compare the detection rate ( DR ) of two LAMP assays versus PCR for the detection of Ca PV . Animals This study used 105 apparently health animals ( AHA ) and 59 clinically sick animals ( CSA ). Methods and materials PCR and LAMP assays ( LAMP 1 and LAMP 2) were compared for detection of Ca PV from AHA and CSA using blood and tissue samples. The detection was confirmed by sequencing of PCR positive samples. Analytical sensitivity and specificity of LAMP assays also were assessed. Results The DR in CSA was 13.6% for PCR whereas for LAMP it was 39.0% and 25.4% for LAMP 1 and 2 methods, respectively. In AHA , the LAMP assay DR was 14.3% and 1.9% for LAMP 1 and 2, respectively. Phylogenetic tree analysis confirmed the identity of Ca PV . Analytic sensitivity showed a detection limit of 8 copies/μL. The analytic specificity test showed no cross detection with other infectious agents. Conclusion and clinical importance Good sensitivity and specificity results for LAMP assay support its application in the routine diagnosis of LSD , whereas its ability to detect LSDV in apparently healthy animals shows its usefulness in identifying populations at risk of LSD .