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Identification of VIM ‐2 metallo‐β‐lactamase‐producing Pseudomonas aeruginosa isolated from dogs with pyoderma and otitis in Korea
Author(s) -
Hyun JaeEun,
Chung TaeHo,
Hwang CheolYong
Publication year - 2018
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/vde.12534
Subject(s) - microbiology and biotechnology , multilocus sequence typing , pseudomonas aeruginosa , biology , aztreonam , colistin , piperacillin , integron , antibiotic resistance , genotype , antibiotics , imipenem , bacteria , genetics , gene
Background Pseudomonas aeruginosa is a challenging pathogen cultured from cases of acute and chronic canine otitis and sometimes in cases of deep pyoderma. The spread of antimicrobial resistance, especially carbapenem resistance, is a serious therapeutic challenge worldwide. Hypothesis/Objectives To investigate the identification and characterization of resistant P. aeruginosa clinical canine isolates. Materials Clinical isolates (n = 80) were collected from dogs with pyoderma (n = 18) and otitis (n = 62) in Korea. Methods Antimicrobial susceptibility was determined using agar dilution and using Clinical and Laboratory Standards Institute guidelines for recording susceptibility for human Pseudomonas isolates; genetic relatedness of isolates was investigated by multilocus sequence typing ( MLST ) and Spe I macrorestriction analysis. The class 1 integrons were amplified and sequenced using primer walking. Results Most isolates were susceptible to colistin (97.5%), polymyxin B (96.3%), ciprofloxacin (81.3%) and meropenem (80.0%); whereas resistance to aztreonam (80%), piperacillin (52.5%), piperacillin/tazobactam (41.3%) and cefepime (37.5%) was high; 12 carbapenem‐nonsusceptible isolates (15%) were detected. MLST revealed 45 different sequence types ( ST s) and macrorestriction analysis detected 55 distinct pulsotypes ( PT s), which were divided into 25 clonal groups. Among carbapenem‐nonsusceptible isolates, 10 (83.3%) were VIM ‐2‐producing strains. Nine VIM ‐2‐producing isolates were identified as ST 1047 and harboured the same 2.8 kb class 1 integron. One remaining isolate was ST 1203 with 2.1 kb class 1 integron. Conclusions and clinical importance This study demonstrated the diversity of the phenotype and genotype of clinical P. aeruginosa isolates from dogs with pyoderma and otitis. The identification of VIM ‐2‐producing P. aeruginosa in dogs is alarming and warrants further surveillance.