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Ultramicronized palmitoylethanolamide counteracts the effects of compound 48/80 in a canine skin organ culture model
Author(s) -
Abramo Francesca,
Lazzarini Giulia,
Pirone Andrea,
Lenzi Carla,
Albertini Sonia,
della Valle M. Frederica,
Schievano Carlo,
Vannozzi Iacopo,
Miragliotta Vincenzo
Publication year - 2017
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/vde.12456
Subject(s) - degranulation , histamine , palmitoylethanolamide , compound 48/80 , mast cell , keratinocyte , skin biopsy , pharmacology , atopic dermatitis , immunology , chemistry , medicine , biopsy , pathology , in vitro , biochemistry , antagonist , receptor , cannabinoid receptor
Background Ultramicronized palmitoylethanolamide ( PEA ‐um) has been reported to reduce pruritus and skin lesions in dogs with moderate atopic dermatitis and pruritus. Hypothesis/Objectives A canine ex vivo skin model was used to investigate the ability of PEA ‐um to counteract changes induced by compound 48/80, a well‐known secretagogue that causes mast cell degranulation. Animals Normal skin was obtained from three donor dogs subjected to surgery for reasons unrelated to the study. Methods Cultured skin biopsy samples in triplicate were treated with 10 and 100 μg/mL compound 48/80, without or with 30 μM PEA ‐um. Mast cell (MC) degranulation, histamine release into the culture medium, local microvascular dilatation, epidermal thickness, keratinocyte proliferation and epidermal differentiation markers were evaluated. Results Exposure of the skin organ culture to PEA ‐um 24 h before and 72 h concomitantly to compound 48/80 resulted in a significant decrease of degranulating MCs. PEA ‐um also reduced the histamine content in the culture medium by half, although the effect did not reach statistical significance. PEA ‐um significantly counteracted vasodilation induced by 100 μg/mL compound 48/80. Finally, PEA ‐um alone did not induce changes in epidermal thickness, differentiation markers, keratinocyte proliferation, MC density and/or degranulation. Conclusions and clinical importance Collectively, these results support the protective action PEA ‐um on the skin of dogs undergoing allergic changes.