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In vitro susceptibility testing of Malassezia pachydermatis to gentamicin
Author(s) -
Silva Freddy A.,
Ferrer Otilia,
Déniz Soraya,
Rosario Inmaculada,
CondeFelipe Magnolia,
Díaz Esther L.,
AcostaHernández Begoña
Publication year - 2017
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/vde.12427
Subject(s) - gentamicin , microbiology and biotechnology , broth microdilution , minimum inhibitory concentration , malassezia , antibiotics , biology
Background Two studies have observed that growth media containing gentamicin can inhibit the growth of the yeast organism Malassezia pachydermatis . The minimum inhibitory concentration ( MIC ) of this bactericidal antibiotic for this organism has not been previously determined. Objective To evaluate the susceptibility of M. pachydermatis isolates to gentamicin. Methods The MIC of gentamicin was determined using a modified version of the M27‐A3 microdilution method following the guidelines of the Clinical and Laboratory Standards Institute. A modified Christensen's urea broth was used to enhance the growth of the M. pachydermatis isolates. Visual and spectrophotometric end‐point readings were performed to detect the presence or absence of yeast growth. Results The MIC 50 and MIC 90 of gentamicin were 8.12 μg/mL and 32.5 μg/mL, respectively; M. pachydermatis strains were classified as susceptible (S), intermediate (I) and resistant (R). The susceptibility of these isolates to gentamicin in vitro, by visual and spectrophotometric end‐point reading, was: S, 54–56%; I, 40–41%; and R, 3–6%. Conclusion Prospective MICs for M. pachydermatis have been established for gentamicin.