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Western blot analysis of sera from dogs with suspected food allergy
Author(s) -
Favrot Claude,
Linek Monika,
Fontaine Jacques,
Beco Luc,
Rostaher Ana,
Fischer Nina,
Couturier Nicolas,
Jacquenet Sandrine,
Bihain Bernard E.
Publication year - 2017
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/vde.12412
Subject(s) - western blot , food allergy , medicine , allergy , gynecology , veterinary medicine , immunology , biology , genetics , gene
Background Food allergy is often suspected in dogs with clinical signs of atopic dermatitis. This diagnosis is confirmed with an elimination diet and a subsequent challenge with regular food. Laboratory tests for the diagnosis of food allergy in dogs are unreliable and/or technically difficult. Cyno‐ DIAL ® is a Western blot method that might assist with the selection of an appropriate elimination diet. Hypothesis/Objectives To evaluate the performance of Cyno‐ DIAL ® for the selection of an elimination diet and diagnosis of food allergy. Animals/Methods Thirty eight dogs with atopic dermatitis completed an elimination diet. Combining the results of the diet trials and the challenges, 14 dogs were classified as food allergic ( FA ), 22 as nonfood‐allergic and two as ambiguous cases. Results Amongst all dogs and amongst dogs with a clinical diagnosis of FA , 3% and 7% (respectively) were positive to Royal Canin Anallergenic ® , Vet‐Concept Kanguru ® or Vet‐Concept Dog Sana ® ; 8% and 7% to Hill's d/d Duck and Rice ® ; 8% and 21% to Hill's z/d Ultra Allergen Free ® ; 53% and 64% to Eukanuba Dermatosis FP ® ; and 32% and 43% to a home‐cooked diet of horse meat, potatoes and zucchini. The specificity and sensitivity of Cyno‐ DIAL ® for diagnosing food allergy were 73% and 71%, respectively. Conclusions and clinical importance Although Cyno‐ DIAL ® was considered potentially useful for identifying appropriate foods for elimination diet trials, it cannot be recommended for the diagnosis of food allergy. The Cyno‐ DIAL ® test performed better than some previously evaluated ELISA ‐based tests.