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Overexpression of TLR ‐2 and TLR ‐4 mRNA in feline polymorphonuclear neutrophils exposed to Microsporum canis
Author(s) -
Cambier Ludivine C.,
Heinen MariePierre A.L.,
Bagut Elena T.,
Antoine Nadine A.,
Mig Bernard R.
Publication year - 2016
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/vde.12295
Subject(s) - microsporum canis , microbiology and biotechnology , receptor , canis , biology , toll like receptor , immune system , pattern recognition receptor , messenger rna , innate immune system , pathogen , immunology , gene , antifungal , paleontology , biochemistry
Background Polymorphonuclear neutrophils ( PMN s), along with macrophages, are the first leukocytes recruited to the site of infection in dermatophytoses and are responsible for the in fine elimination of the fungus. It has been demonstrated that feline PMN s produce pro‐inflammatory cytokines after stimulation with Microsporum canis . The activation of these cells results from the recognition of specific PAMP s (pathogen associated molecular patterns) from M. canis by PRR s (pattern recognition receptors) of PMN s. The C‐type lectin receptors ( CLR s) and toll‐like receptors ( TLR s) are the two main PRR s in phagocytic cells that recognize fungal components. Hypothesis/Objective The aim of this study was to evaluate the expression of TLR ‐2, TLR ‐4 and dectin‐1 mRNA in feline PMN s exposed to different components from M. canis . Methods Feline PMN s were stimulated for 2 h or 4 h with either live arthroconidia, heat‐killed arthroconidia or secreted components from M. canis . The levels of TLR ‐2, TLR ‐4 and dectin‐1 mRNA were assessed by RT ‐q PCR . Results Results showed an increase of TLR ‐2 and TLR ‐4 mRNA levels in feline PMN s stimulated with live and heat‐killed arthroconidia, but not in those stimulated with the secreted components from M. canis . No significant variation in dectin‐1 mRNA expression was observed in PMN s stimulated with the different fungal components. Conclusions and clinical importance The overexpression of TLR ‐2 and TLR ‐4 mRNA s in stimulated feline PMN s suggests that these receptors are involved in the host immune response through the recognition of M. canis PAMP s.