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Increased numbers of FoxP3‐expressing CD 4 + CD 25 + regulatory T cells in peripheral blood from dogs with atopic dermatitis and its correlation with disease severity
Author(s) -
Hauck Verena,
Hügli Patrick,
Meli Marina L.,
Rostaher Ana,
Fischer Nina,
HofmannLehmann Regina,
Favrot Claude
Publication year - 2016
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/vde.12279
Subject(s) - foxp3 , atopic dermatitis , medicine , peripheral blood mononuclear cell , pathogenesis , il 2 receptor , immunology , flow cytometry , regulatory t cell , immune system , peripheral blood , disease , t cell , biology , biochemistry , in vitro
Background Atopic dermatitis ( AD ) is a common chronic inflammatory skin disease of humans and dogs. Regulatory T cells (Tregs) are essential controllers of immune homeostasis and have been shown to play a key role in human AD , even though frequencies of Tregs in atopic human patients vary greatly. Only two studies have reported Treg numbers in the peripheral blood of dogs with canine AD (CAD). Objectives This study aimed to assess the numbers of circulating Tregs in healthy and atopic dogs, and to determine whether Treg numbers correlate with age, sex, disease severity or pre‐treatment. Animals Client‐owned dogs including 14 healthy dogs and 35 dogs with CAD . Methods Expression of Tregs in peripheral blood mononuclear cells was evaluated by flow cytometry. Tregs were phenotypically identified as T cells triple positive for CD 4, CD 25 and FoxP3. Results The percentage of circulating CD 4 + CD 25 + FoxP3 + Tregs in atopic dogs was increased significantly compared to healthy dogs (mean 2.1% versus 1%, P = 0.002) and correlated with disease severity (Pruritus Scale: r = 0.48, P = 0.003; CADESI ‐04: r = 0.34, P = 0.044). No significant differences in age or sex were found in either group and pre‐treatment had no influence on results for atopic dogs. Conclusions Data suggest that, as in humans, CD 4 + CD 25 + FoxP3 + Tregs may contribute to the pathogenesis of CAD as indicated by an association between Treg frequency and disease severity. Further investigation is required to improve the understanding of the role of Tregs in atopic dogs.