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Serum concentration and skin tissue expression of insulin‐like growth factor 2 in canine generalized demodicosis
Author(s) -
Yarim Gul F.,
Yagci Bugrahan B.,
Yarim Murat,
Sozmen Mahmut,
Pekmezci Didem,
Cenesiz Sena,
Pekmezci Gokmen Z.,
Karaca Efe
Publication year - 2015
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/vde.12270
Subject(s) - demodicosis , medicine , dermis , immunohistochemistry , pathology , endocrinology , dermatology
Background There is increasing evidence that insulin‐like growth factor‐2 ( IGF ‐2) levels are altered in skin injury; there are no data evaluating the serum concentration and skin tissue expression of IGF ‐2 in canine generalized demodicosis. Hypothesis/Objectives To assess serum concentrations of IGF ‐2 collected from dogs with generalized demodicosis compared to healthy dogs and to determine the location of IGF ‐2 in the skin of affected dogs. Methods Blood and skin samples were collected from 12 dogs of differing breeds and gender at 1–2 years of age that had a confirmed diagnosis of generalized demodicosis. Age‐matched control skin and blood samples were collected from 11 normal dogs of different breeds and gender. Serum IGF ‐2 concentrations were measured by enzyme‐linked immunosorbent assay. Skin tissue expression of IGF ‐2 was analysed by immunohistochemical methods. Results Serum concentration and skin tissue expression of IGF ‐2 were increased in dogs with generalized demodicosis compared with control dogs. Conclusions and clinical importance These findings indicate that keratinocytes, histiocytes and fibrocytes in the dermis are positive for IGF ‐2; they may be a source of the elevated serum IGF ‐2 levels in dogs with generalized demodicosis.