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Preferential gene transcription of T helper 2 cytokines in peripheral CCR4 + CD4 + lymphocytes in dogs
Author(s) -
Iio Aki,
Motohashi Tsutomu,
Kunisada Takahiro,
Yasuhira Yuma,
Kamishina Hiroaki,
Maeda Sadatoshi
Publication year - 2014
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/vde.12125
Subject(s) - ccr4 , immunology , pathogenesis , chemokine , cc chemokine receptors , atopic dermatitis , interleukin 4 , interferon , interleukin , medicine , biology , chemokine receptor , cytokine , inflammation
Background Previous studies reported the involvement of CC chemokine receptor 4 (CCR4)‐positive CD4 + cells in the pathogenesis of canine atopic dermatitis. In humans, CCR4 is selectively expressed on type 2 helper T (Th2) cells; however, a subset of canine CCR4 + helper T cells has not been determined. Hypothesis/Objectives To characterize the transcription profile of CCR4 + CD4 + lymphocytes isolated from the peripheral blood of healthy dogs. Animals Three healthy dogs were used. Methods The transcription levels of type 1 helper T (Th1) and Th2 cytokines in CCR4 + CD4 + and CCR4 − CD4 + lymphocytes isolated from healthy dogs were quantified by real‐time RT‐PCR. Results The CCR4 + CD4 + lymphocytes preferentially transcribed Th2 cytokines, such as interleukin‐4 and interleukin‐13, but not Th1 cytokines, such as interferon‐γ. Conclusions and clinical importance CCR4 can be used as a specific marker of Th2 cells for elucidation of the pathogenesis or the establishment of novel therapeutics in canine Th2‐associated diseases, such as canine atopic dermatitis.