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Equine intradermal test threshold concentrations for house dust mite and storage mite allergens and identification of stable acari fauna
Author(s) -
Roberts Holly A.,
Hurcombe Samuel D. A.,
Hillier Andrew,
Lorch Gwendolen
Publication year - 2014
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/vde.12113
Subject(s) - mite , tyrophagus putrescentiae , fauna , acari , biology , veterinary medicine , pyroglyphidae , house dust mite , immunoglobulin e , zoology , ecology , medicine , immunology , antibody
Background House dust mite ( HDM ) and storage mite ( SM ) stable fauna and their associated equine intradermal test ( IDT ) threshold concentrations ( TC s) for the midwestern region of the USA are unknown. Hypothesis/Objectives To determine IDT TC s and serum I g E concentrations for two HDM and three SM species in clinically normal horses over two seasons, and to identify the mite taxa and habitats in a stable. Animals Thirty‐eight clinically normal horses. Methods Threshold concentrations for HDM s and SM s were determined using IDT subjective measurements and a statistical model. An enzyme‐linked immunosorbent assay was used to quantify serum I g E concentrations for the same mite species. A modified flotation method was used to identify morphologically HDM s and SM s. Results Subjective IDT TC s were as follows: 1:80,000 w/v for Dermatophagoides farinae in both seasons; 1:80,000 w/v in spring and 1:160,000 w/v in late summer for Dermatophagoides pteronyssinus ; 1:40,000 w/v in spring and 1:20,000 w/v in late summer for Acarus siro ; 1:20,000 w/v for Lepidoglyphus destructor in both seasons; and 1:20,000 w/v in spring and 1:10,000 w/v in late summer for Tyrophagus putrescentiae . Statistically significant associations for increased serum IgE and a positive IDT reaction were evident for D. farinae in the spring and D. pteronyssinus in both seasons. One mite from all four genera specific to this study was identified; however, two HDM and A. siro species were not detected. Conclusions and clinical importance This study established HDM and SM IDT dilution concentrations for the horses in this region. Exposure to diverse acaridae fauna may contribute to the pathogenesis of equine allergic disease.