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Transcription profile of chemokine receptors, cytokines and cytotoxic markers in peripheral blood of dogs with epitheliotropic cutaneous lymphoma
Author(s) -
Chimura Naoki,
Iio Aki,
Ozaki Eiji,
Mori Takashi,
Ito Yusuke,
Murayama Nobuo,
Nagata Masahiko,
Ide Kaori,
Nishifuji Koji,
Kamishina Hiroaki,
Maeda Sadatoshi
Publication year - 2013
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/vde.12076
Subject(s) - cytotoxic t cell , granzyme b , chemokine , immunology , chemokine receptor , cxcr3 , medicine , granulysin , cc chemokine receptors , biology , pathology , perforin , immune system , t cell , cd8 , in vitro , biochemistry
Background Mycosis fungoides ( MF ) is the most common form of canine epitheliotropic cutaneous lymphoma, which is characterized by the accumulation of neoplastic CD 8 + T cells. Given that multifocal skin lesions are commonly seen in MF , neoplastic lymphocytes may actively migrate into the blood circulation. Hypothesis/Objectives Cytotoxic T cells with a skin‐homing phenotype could be increased in the blood circulation of dogs with MF . Animals Ten dogs with MF and 10 age‐matched healthy dogs were included. Methods The transcription levels of chemokine receptors, cytokines and cytotoxic markers in peripheral blood of dogs with MF were quantified by real‐time RT ‐ PCR . Results The dogs with MF had lower transcription levels of chemokine receptors associated with skin homing ( CCR 4), epitheliotropism ( CXCR 3), lymph node homing ( CCR 7), a type‐1 cytokine ( LT ‐α) and cytotoxic markers (perforin and granzyme B ) in the circulation than healthy control dogs ( P < 0.05). Conclusions and clinical importance The present results suggest that the number of peripheral cytotoxic T cells with a skin‐homing phenotype could be decreased in the peripheral blood of dogs with MF , which might be due to the sequestration of cytotoxic T cells in the lesional skin.