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In vitro effects of C p G oligodeoxynucleotides delivered by gelatin nanoparticles on canine peripheral blood mononuclear cells of atopic and healthy dogs – a pilot study
Author(s) -
Prélaud Ana Rostaher,
Fuchs Sebastian,
Weber Karin,
Winter Gerhard,
Coester Conrad,
Mueller Ralf S.
Publication year - 2013
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/vde.12056
Subject(s) - peripheral blood mononuclear cell , cpg oligodeoxynucleotide , medicine , cpg site , tlr9 , cytokine , immunology , in vitro , in vivo , interleukin , biology , dna methylation , biochemistry , gene expression , microbiology and biotechnology , gene
Background Cytosine‐phosphate‐guanine ( C p G ) oligodeoxynucleotides offer a novel promising immunotherapeutic approach for atopic dermatitis ( AD ) both in humans and animals. Gelatin nanoparticles ( GNP ) enhance and prolong C p G ‐associated immunomodulatory effects and minimize adverse effects both in vitro and in vivo . Information about the effects of this combination in dogs is lacking. Hypothesis/Objectives The aim of this study was to evaluate immunological effects of C p G coupled to GNP on canine peripheral blood mononuclear cells ( PBMC s) in vitro . Animals Eight dogs with AD , diagnosed by standard criteria and with a concurrent immediate hypersensitivity to house dust mites were included. Control samples were taken from eight healthy, age‐matched control dogs without history or evidence of cutaneous or systemic illness. Methods Peripheral blood mononuclear cells of healthy and allergic dogs were incubated with CpG‐ GNP and the uptake of CpG‐ GNP was demonstrated using confocal laser scanning microscopy. Cell culture supernatant concentrations of interferon gamma ( IFN ‐γ), interleukin ( IL )‐4, IL ‐6 and IL ‐10 were measured by Canine Cytokine Milliplex. Results No significant changes in IFN ‐γ and IL ‐4 were found when comparing PBMC s incubated with CpG and CpG‐ GNP with the negative controls in atopic and healthy dogs. Interleukin‐6 was not detected in any of the groups. However, a statistically significant increase in IL ‐10 concentration was found after 24 h stimulation with CpG‐ GNP compared with CpG alone both in atopic and healthy dogs. Conclusions and clinical importance As IL ‐10 is considered an immunosuppressive cytokine playing a key role in peripheral tolerance; the reported CpG‐ GNP formulation could be a new approach in allergy treatment.