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In vitro efficacy of the essential oil from Leptospermum scoparium (manuka) on antimicrobial susceptibility and biofilm formation in Staphylococcus pseudintermedius isolates from dogs
Author(s) -
Song ChiYoun,
Nam EuiHwa,
Park SeolHee,
Hwang CheolYong
Publication year - 2013
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/vde.12045
Subject(s) - staphylococcus pseudintermedius , manuka honey , antimicrobial , microbiology and biotechnology , agar dilution , essential oil , biofilm , agar , biology , staphylococcus aureus , minimum inhibitory concentration , staphylococcus , bacteria , food science , genetics
Background Staphylococcus pseudintermedius is a common pathogen of skin and ear infections in dogs. The widespread and rapid emergence of meticillin‐resistant S. pseudintermedius ( MRSP ) has created therapeutic challenges in veterinary medicine and the need for alternative treatments. Hypothesis/Objectives We aimed to evaluate the in vitro antimicrobial activity of the essential oil manuka ( Leptospermum scoparium ) against S. pseudintermedius . Methods This study was performed using S. pseudintermedius strains isolated from dogs with skin and ear infections collected throughout Korea between 2009 and 2011. The in vitro antimicrobial activity of manuka oil against 39 MRSP and 11 meticillin‐susceptible S. pseudintermedius ( MSSP ) strains was analysed by measuring minimal inhibitory concentrations ( MIC s) using the agar dilution method and biofilm inhibition activity as assessed by the colorimetric microtitre plate assay. Results Our results indicated that manuka oil had excellent activity against all bacterial isolates. The MIC s for MRSP and MSSP to manuka oil were in the range of 2 −9 to 2 −6 and 2 −9 to 2 −7 % (v/v), respectively. Manuka oil was a potent inhibitor of S. pseudintermedius biofilm formation, and the majority of bacteria decreased by >50%. No significant differences were observed in the MIC s or biofilm formation between the MRSP and MSSP strains. Conclusions and clinical importance These results suggest that manuka oil has the potential to be a useful therapeutic option for treating superficial infections caused by MRSP and MSSP ; further clinical investigations are required.