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Study to assess in vitro antimicrobial activity of nine ear cleaners against 50 M alassezia pachydermatis isolates
Author(s) -
Mason Carly L.,
Steen Stephen I.,
Paterson Susan,
Cripps Peter J.
Publication year - 2013
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1111/vde.12024
Subject(s) - malassezia , otitis , antimicrobial , microbiology and biotechnology , medicine , in vitro , agar , biology , bacteria , surgery , biochemistry , genetics
Background Ear cleaning is an important part of the successful therapy of otitis externa. Hypothesis/Objectives To evaluate the in vitro activity of nine commercially available ear cleaners ( C erumaural ® , C lean A ural D og ® , E pi‐ O tic ® , M al A cetic A ural ® , O toclean ® , O todine ® , S ancerum ® , S urosolve ® and T riz U ltra™ + K eto) against 50 M alassezia pachydermatis isolates from clinical cases of canine otitis externa. To the authors' knowledge, this is the first study to analyse the in vitro susceptibility of a large number of M . pachydermatis isolates to a variety of ear cleaner products. Animals Forty‐nine hospital population dogs diagnosed with otitis externa with M alassezia spp. present on cytological examination of ear swabs and culture. Methods The antimicrobial activity of nine ear cleaners was tested against 50 M . pachydermatis isolates from 49 clinical cases of canine otitis externa using an in vitro agar diffusion test. Results There were statistically significant differences in activity between ear cleaners, with five showing excellent in vitro anti‐ M alassezia activity ( C lean A ural D og ® , E pi‐ O tic ® , M al A cetic A ural ® , S ancerum ® and T riz U ltra™ + K eto), two moderate activity ( O todine ® and S urosolve ® ), one variable activity ( O toclean ® ) and one no activity ( C erumaural ® ). There was a significant overall difference in susceptibility between M . pachydermatis isolates tested ( P < 0.001). Conclusions and clinical importance Ear cleaners with activity against M alassezia may help to reduce the unnecessary use of antibiotic‐containing polypharmaceutical ear medications. This study shows that the use of a large number of isolates may provide a more accurate account of the in vitro activity of a product.