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Impact of blood storage duration on hematologic, blood gas, biochemical, and oxidative stress variables in sheep undergoing allogeneic blood transfusions
Author(s) -
Sousa Rejane S.,
Minervino Antonio H. H.,
Oliveira Francisco Leonardo C.,
Araújo Carolina Akiko Sato C.,
Rodrigues Frederico Augusto M. L.,
Zaminhan Janayna Larissa R.,
Vale Rodolfo G.,
Tavares Marcondes D.,
Mori Clara S.,
Paula Valéria V.,
Ortolani Enrico L.,
Barrêto Júnior Raimundo A.
Publication year - 2020
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12917
Subject(s) - hemoglobin , medicine , hemolysis , phlebotomy , blood transfusion , hematocrit , whole blood , red blood cell , oxidative stress , hemotherapy , autotransfusion , leukoreduction , physiology , andrology
Background Hemotherapy in ruminants is limited to whole blood transfusions, sometimes with stored blood for up to 42 days, but little attention has been given to the effect of blood storage times and recipient responses after transfusions. Objectives We aimed to evaluate the hematologic and serum biochemical effects after allogeneic blood transfusion with either fresh or stored blood in sheep. We also sought to examine hematologic and biochemical analyte changes in the store blood. Methods Eighteen sheep underwent a single phlebotomy to remove 40% of their blood volume. The sheep were divided into three experimental groups, G0, G15, and G35, which included six animals, each receiving 20 mL/kg of either fresh blood or blood stored in citrate, phosphate, dextrose, and adenine (CPDA‐1) bags for 15 and 35 days, respectively. Biochemical, hematologic, coagulation, blood gas, lipid peroxidation, and oxidative stress test evaluations were performed using the blood samples gathered at T0 (before transfusion), 30 minutes (T30m), 6, 12, 24, 48, 72, and 96 hours (T6h‐T96h), 8 days (T8d), and 16 days (T16d) after transfusions. Results Sheep exhibited increases in packed cell volumes, red blood cell counts, and total hemoglobin concentrations at T30m ( P < .05). G35 animals had greater plasma hemoglobin concentrations at T12h and decreased blood pH values at T6h, characterized by slight metabolic acidemia. Regarding oxidative stress, G35 animals had decreased catalase activities from T0 at T30m, T6h, T12h, and T24h, indicating that hemolysis had occurred, which was supported by concomitant increases in bilirubin. Conclusions Sheep transfused with 35‐day stored blood exhibited greater hematologic, blood gas, biochemical, and oxidative alterations; however, anemic animals without comorbidities effectively reversed those alterations.