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Precision and accuracy of the Mindray BC‐5000Vet hematology analyzer for canine and feline blood
Author(s) -
Thongsahuan Sorawat,
Fonghoi Lalita,
Kaewfai Sunita,
Srinoun Kanitta
Publication year - 2020
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12861
Subject(s) - hematology analyzer , hematology , cats , coefficient of variation , white blood cell , medicine , complete blood count , blood cell , whole blood , hemoglobin , pathology , chemistry , chromatography
Background The Mindray BC‐5000Vet hematology analyzer is a flow cytometry‐based automated hematology analyzer that generates a complete blood count with a five‐part white blood cell (WBC) differential count. Objectives We aimed to validate reliability results of the Mindray BC‐5000Vet for use in dog and cat blood. Methods Imprecision was performed using the manufacturer’s quality control material at low, normal, and high levels. Blood sample results of healthy and ill dogs and cats were studied for comparability between manual methods and the Mindray BC‐5000Vet analyzer. Forty dogs and 40 cats were included in the study. Results Precision for red blood cell (RBC) parameters was excellent, with a coefficient of variation within‐run (%CV w ) and between‐run (%CV b ) of <2%. WBC count and differential count showed %CV w and %CV b <8%; however, %CV w and %CV b of low‐level control material gave >9% eosinophils. The correlation between the BC‐5000 Vet and manual methods in normal and abnormal canine and feline blood samples showed excellent correlations for the RBC counts, hemoglobin concentrations, hematocrits, and WBC counts ( r > .93). The differential WBC analysis of canine blood showed good correlation ( r = .80‐.92). Feline blood samples showed excellent correlations for neutrophils and lymphocytes, with a good correlation for monocytes and eosinophils. Conclusions The Mindray BC‐5000Vet hematology analyzer proved a suitable instrument for routine analysis in dogs and cats with various hematologic abnormalities.