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Method validation, reference values, and characterization of acute‐phase protein responses to experimentally induced inflammation and bluetongue virus infection in the Iberian ibex
Author(s) -
Pastor Josep,
Bach Ester,
RáezBravo Arián,
LópezOlvera Jorge Ramón,
Tvarijonaviciute Asta,
Granados José Enrique,
Espinosa José,
Pérez Jesús,
Lavín Santiago,
Cuenca Rafaela
Publication year - 2019
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12802
Subject(s) - haptoglobin , serum amyloid a , acute phase protein , asymptomatic , inflammation , coefficient of variation , virus , medicine , biology , immunology , chemistry , chromatography
Background Acute phase protein (APP) concentrations can change due to inflammation and be used to monitor disease in the Iberian ibex ( Capra pyrenaica ). Objectives This study aimed to validate Haptoglobin (Hp) and serum amyloid A (SAA) analytes, establish reference values, and characterize Hp and SAA responses in the Iberian ibex after experimentally induced inflammation and experimental bluetongue virus (BTV) infection. Methods Sera from 40 free‐ranging box‐trapped ibexes were used to establish Hp and SAA reference values. Six healthy ibexes were subcutaneously injected with 5 mL of turpentine, then, blood samples were taken, and clinical evaluations were performed on days 0, 1, 2, 3, 4, 7, and 14 postinjection. Another seven ibexes were challenged with BTV. Serum Hp and SAA concentrations were quantified using commercial assays following the manufacturer's instructions. Results Intra‐assay precision and linearity were acceptable for both Hp and SAA. Intra‐assay variation for high and low concentration of Hp and SAA were 9.74% and 17.31% and 16.49% and 12.89%, respectively. Inter‐assay variation was higher for the low APP concentrations. Reference values for the healthy Iberian ibexes were (median, minimum, and maximum values) 0.2 (0.12‐0.64) g/L for Hp and 4.74 (0.05‐29.54) mg/L for SAA. Both Hp and SAA acted as a moderate and a major APP, respectively, and each could distinguish animals with turpentine‐induced inflammation from those without. Hp and SAA did not change in asymptomatic BTV‐infected animals. Conclusion This study validated Hp and SAA analytes and provided basal reference values for these analytes in the Iberian ibex. Both APPs were able to discriminate between healthy and diseased Iberian ibexes animals during turpentine‐induced inflammatory processes.