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Validation of an ELISA for detection of neutrophil gelatinase‐associated lipocalin (NGAL) in equine serum
Author(s) -
Jacobsen Stine,
Berg Lise C.,
Tvermose Emil,
Laurberg Malene B.,
van Galen Gaby
Publication year - 2018
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12670
Subject(s) - lipocalin , creatinine , gelatinase , neutrophil gelatinase associated lipocalin , turbidimetry , coefficient of variation , medicine , serum concentration , andrology , immunology , chemistry , chromatography , matrix metalloproteinase
Background Neutrophil gelatinase‐associated lipocalin (NGAL) has been shown to be a useful marker of kidney injury in people and dogs, but has not been described in horses. Objectives The aim of the study was to validate a commercially available porcine‐specific ELISA to measure serum concentrations of equine NGAL. Methods Intra‐ and interassay imprecisions were evaluated by multiple measurements on equine serum pools. Assay inaccuracy was determined by the linearity under dilution. Overlapping performance was assessed by measuring NGAL concentrations in horses with normal and elevated serum creatinine levels. Results Intra‐ and interassay imprecision (coefficient of variation) ranged from 5.35% to 28.39%. The ELISA showed no signs of inaccuracy. Overlapping performance was acceptable, as the assay was able to detect the expected differences of NGAL levels in horses with normal and elevated serum creatinine concentrations. Conclusions Equine serum NGAL concentrations could be quantified reliably using the porcine‐specific ELISA.