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Reproducibility, stability, and biological variability of thrombin generation using calibrated automated thrombography in healthy dogs
Author(s) -
Cuq Benoît,
Blois Shauna L.,
Wood R. Darren,
Monteith Gabrielle,
AbramsOgg Anthony C.,
Bédard Christian,
Wood Geoffrey A.
Publication year - 2018
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12602
Subject(s) - venipuncture , thrombin generation , reproducibility , repeatability , medicine , hemostasis , anesthesia , thrombin , chemistry , platelet , chromatography
Background Thrombin plays a central role in hemostasis and thrombosis. Calibrated automated thrombography ( CAT ), a thrombin generation assay, may be a useful test for hemostatic disorders in dogs. Objectives To describe CAT results in a group of healthy dogs, and assess preanalytical variables and biological variability. Animals Forty healthy dogs were enrolled. Methods Lag time ( Lag ), time to peak ( ttpeak ), peak thrombin generation ( peak ), and endogenous thrombin potential ( ETP ) were measured. Direct jugular venipuncture and winged‐needle catheter‐assisted saphenous venipuncture were used to collect samples from each dog, and results were compared between methods. Sample stability at −80°C was assessed over 12 months in a subset of samples. Biological variability of CAT was assessed via nested ANOVA using samples obtained weekly from a subset of 9 dogs for 4 consecutive weeks. Results Samples for CAT were stable at −80°C over 12 months of storage. Samples collected via winged‐needle catheter venipuncture showed poor repeatability compared to direct venipuncture samples; there was also poor agreement between the 2 sampling methods. Intra‐individual variability of CAT parameters was below 25%; inter‐individual variability ranged from 36.9% to 78.5%. Conclusions Measurement of thrombin generation using CAT appears to be repeatable in healthy dogs, and samples are stable for at least 12 months when stored at −80°C. Direct venipuncture sampling is recommended for CAT . Low indices of individuality suggest that subject‐based reference intervals are more suitable when interpreting CAT results.