Premium
Preparation of canine C‐reactive protein serum reference material: A feasibility study
Author(s) -
Canalias Francesca,
Piñeiro Matilde,
Pato Raquel,
Peña Raquel,
Bosch Lluís,
Soler Lourdes,
García Natalia,
Lampreave Fermín,
Saco Yolanda,
Bassols Anna
Publication year - 2018
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12574
Subject(s) - chromatography , bovine serum albumin , chemistry , matrix (chemical analysis)
Background The availability of a species‐specific reference material is essential for the harmonization of results obtained in different laboratories by different methods. Objectives We describe the preparation of a canine C‐reactive protein ( cCRP ) serum reference material containing purified cCRP stabilized in a serum matrix. The material can be used by manufacturers to assign values to their calibrator and control materials. Methods The serum matrix was obtained using blood collected from healthy dogs, stabilized and submitted for a delipidation process. The reference material was prepared by diluting purified cCRP in the serum matrix containing 1.0 mol/L HEPES buffer, 3.0 mmol/L calcium chloride, 80,000 kUI /L aprotinin, and 1.0 mmol/L benzamidine hydrochloride monohydrate at a pH of 7.2, and dispensing (0.5 mL ) the matrix into vials that were then frozen. Results The pilot batch of 200 vials was shown to be homogeneous and stable after a stability study at various temperatures and over a total time of 110 days. The prepared material was submitted to an assignment value study. Eight laboratories from different European countries participated by using the same reagents for an immunoturbidimetric method adapted for different analyzers. The obtained cCRP concentration in the reference material was 78.5 mg/L with an expanded uncertainty ( k = 2) of 4.2 mg/L. Conclusions Canine C‐reactive protein serum reference material has been produced that allows harmonization of results obtained by different methods and different laboratories, thus reducing the possibility of errors and misunderstandings.