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Electrolyte, acid–base, and hemoglobin oxygen affinity alterations following irradiation and storage of canine packed red blood cells
Author(s) -
Press Saya A.,
Cooper Edward S.,
Stull Jason W.
Publication year - 2017
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12539
Subject(s) - hemoglobin , irradiation , chemistry , oxygen , blood irradiation therapy , methemoglobin , potassium , red blood cell , biochemistry , andrology , medicine , physics , organic chemistry , nuclear physics
Background Irradiation of RBC before transfusion is required to prevent transfusion‐associated graft‐versus‐host disease for human patients undergoing hematopoietic stem cell transplantation. Additional applications for irradiated blood may exist in oncologic surgery. The effect of irradiation on canine packed RBC ( pRBC ) is unknown. Objectives The aim of this study was to explore and characterize the in vitro electrolyte, acid–base, and oxygen‐carrying capacity changes to pRBC immediately following irradiation and during storage. Methods Ten units of pRBC were irradiated using a linear accelerator. Concentration of potassium and glucose, percentage of free hemoglobin ( fH b), hemoglobin oxygen saturation ( sO 2 ), total oxygen content, partial pressure of oxygen ( pO 2 ), the pO 2 at which 50% of hemoglobin is saturated (p50), lactate, pH , and methemoglobin were measured before and following irradiation, and at 7 and 17 days post irradiation. Results In both irradiated and nonirradiated units, a significant decrease in pH and glucose, and a significant increase in lactate and potassium were noted. The pO 2 , fH b, and the p50 value in both groups increased over the first 7 days. Immediately following irradiation, the pH was significantly lower, and the potassium, lactate, and fH b were significantly higher in irradiated units compared with controls. Small but significant differences were noted between irradiation status in pH , fH b, sO 2 , total oxygen content, and p50 value at 7 days post irradiation. Conclusions This hypothesis‐generating study found irradiation and storage significantly altered in vitro properties of pRBC . The magnitude of these differences was small and the clinical impact of irradiation on pRBC may be negligible.