Premium
Rapid immunocytochemistry for the detection of cytokeratin and vimentin: assessment of its diagnostic value in neoplastic diseases of dogs
Author(s) -
Sawa Mariko,
Inoue Mayuko,
Yabuki Akira,
Kohyama Moeko,
Miyoshi Noriaki,
Setoguchi Asuka,
Yamato Osamu
Publication year - 2017
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12462
Subject(s) - vimentin , immunocytochemistry , cytokeratin , pathology , value (mathematics) , medicine , immunohistochemistry , computer science , machine learning
Background Immunocytochemistry ( ICC ) is an advanced diagnostic technique used in the field of veterinary cytology. We recently developed a rapid ICC method for the detection of cytokeratin and vimentin in dogs, which helps to determine whether tumor cells are of epithelial or nonepithelial origin. However, the diagnostic value of this rapid ICC method in neoplastic diseases of dogs has not been assessed yet. Objectives The aim of the present study was to assess the diagnostic accuracy of rapid ICC compared to standard immunohistochemistry ( IHC ). Methods Air‐dried smear samples and formalin‐fixed paraffin sections were prepared from tumors excised from dogs ( n = 30). Immunosignals for cytokeratin and vimentin were detected in smear samples by rapid ICC , and in paraffin sections by standard IHC . Signals in smear samples detected by rapid ICC were compared with positive staining in paraffin sections detected by standard IHC and analyzed for statistical significance (kappa statistic). Results Rapid ICC detected specific immunosignals in 25/30 cases (83.3%), and nonspecific signals were detected in 5/30 cases. Statistical analysis revealed fair agreement in epithelial tumors ( n = 16) with cytokeratin (κ = 0.236) and vimentin (κ = 0.294). In nonepithelial tumors ( n = 14), almost perfect agreement was demonstrated with cytokeratin (κ = 0.857) and vimentin (κ = 0.857). Conclusions The rapid ICC method can be a useful tool for the diagnostic cytology of neoplastic tissues in dogs.