Premium
Serum creatine kinase isoenzymes and macroenzymes in dogs with different neurologic diseases
Author(s) -
Paltrinieri Saverio,
Pintore Laura,
Balducci Federica,
Giordano Alessia,
Costabile Annaluce,
Bernardini Marco
Publication year - 2017
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12443
Subject(s) - medicine , creatine kinase , pathology , epilepsy , isozyme , gastroenterology , creatine , biology , enzyme , biochemistry , psychiatry
Background Increased serum activity of CK isoenzymes and macroenzymes, and in particular of the brain isoenzyme ( CK ‐ BB ) has been reported in dogs with central nervous system ( CNS ) disorders. However, no studies on the possible differences in serum activities of CK iso‐ or macroenzymes (Macro‐ CK 1 and Macro‐ CK 2) in different neurologic diseases are available. Objective The aim of this study was to describe the electrophoretic distribution of CK iso‐ and macroenzymes in dogs with CNS disorders in order to assess whether this distribution depends on a specific neurologic disease. Methods This study was done on sera from 45 dogs with neurologic diseases (degenerative, n = 7; idiopathic epilepsy [ IE ], n = 14; inflammatory, n = 16; space occupying lesions [ SOL ], n = 8) and from 10 clinically healthy dogs. The separation of serum CK isoenzymes and macroenzymes was performed using an automated electrophoretic method already validated in dogs. Results Compared with healthy dogs, dogs with CNS disorders had significantly higher total CK and CK ‐ BB activities, and a significantly lower Macro‐ CK 2 activity ( P < .001). Comparison of pathologic subgroups and healthy dogs revealed significant differences ( P < .01) in dogs with IE and inflammatory disorders for total CK activity, in all the subgroups for CK ‐ BB ( P < .01), and in dogs with IE and SOL for Macro‐ CK 2 ( P < .01). Conclusions The results of this study suggest that CK ‐ BB is released by neurons damaged by inflammatory or degenerative conditions or due to compressive effects of SOL . However, the neurologic diseases cannot be differentiated based on CK ‐ BB or Macro‐ CK 2 activities, unless further studies allow the definition of diagnostic thresholds.