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Evaluation of hematologic variables in newborn C57/ BL 6 mice up to day 35
Author(s) -
White Jessica R.,
Gong Huiyu,
Colaizy Tarah T.,
Moreland Jessica G.,
Flaherty Heather,
McElroy Steven J.
Publication year - 2016
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12314
Subject(s) - medicine , complete blood count , blood sampling , reference values , physiology , andrology , immunology
Background Hematologic variables are often analyzed in animal analogs during the investigation of complex disease etiologies such as necrotizing enterocolitis. However, reference intervals ( RI ) can vary depending on animal strain, age, and sampling site. Reference intervals have been published for adult C57 BL /6J mice, but not newborn C57 BL /6J mice. Objectives The purpose of the present study was to determine hematologic RI in newborn C57 BL /6J mice up to day 35. Methods C57 BL /6J mice founders from The Jackson Laboratory were bred at the University of Iowa. Blood samples were obtained via facial vein sampling at postnatal days 0 (p0), p7, p14, p21, p28, or young adulthood (p35). CBC s were determined with the Sysmex XT ‐2000iV analyzer within 30 minutes of blood collection at a 1:10 dilution. Statistics were determined using nonparametric methods following ASVCP guidelines. Results Hematologic RI were determined for each of the 6 groups ( n = 247, n ≥ 39 per group). Significantly higher values for HGB , RBC , and PLT counts were observed with advancing developmental age. Total WBC counts remained relatively stable during the first 35 days of life. However, WBC differential counts were dominated by neutrophils and lymphocytes in the younger mice, with a trend toward a lymphocytic leukogram on day 35. Conclusions These results illustrate the dynamic changes in hematologic variables during murine development after birth. Utilization of age‐specific RI is advised when evaluating data derived from experimental perinatal mouse models.