Premium
Reliability of the Lactate Scout point‐of‐care instrument for the determination of blood l ‐lactate concentration in sheep
Author(s) -
Kaynar Ozgur,
Karapinar Tolga,
Hayirli Armagan,
Baydar Ersoy
Publication year - 2015
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12288
Subject(s) - blood lactate , reliability (semiconductor) , point of care , lactic acid , chromatography , chemistry , medicine , pathology , biology , bacteria , physics , heart rate , blood pressure , power (physics) , quantum mechanics , genetics
Background Data on accuracy and precision of the Lactate Scout point‐of‐care (POC) analyzer in ovine medicine are lacking. Objective The purpose of the study was to assess the reliability of the Lactate Scout in sheep. Materials and Methods Fifty‐seven sheep at varying ages with various diseases were included. Blood lactate concentration in samples collected from the jugular vein was measured immediately on the Lactate Scout. Plasma l ‐lactate concentration was measured by the Cobas autoanalyzer as the reference method. Data were subjected to Student's t ‐test, Passing–Bablok regression, and Bland–Altman plot analyses for comparison and assessment of accuracy, precision, and reliability. Results Plasma l ‐lactate concentration was consistently lower than blood l ‐lactate concentration (3.06 ± 0.24 vs 3.3 ± 0.3 mmol/L, P < .0001). There was a positive correlation between plasma and blood l ‐lactate concentrations ( r = .98, P < .0001). The Lactate Scout had 99% accuracy and 98% precision with the reference method. Blood ( Y ) and plasma ( X ) l ‐lactate concentrations were fitted to Y = 0.28 + 1.00 · X , with a residual standard deviation of 0.31 and a negligible deviation from the identity line ( P = .93). The bias was fitted to Y = 0.10 + 0.05 · X , with Sy.x of 0.44 ( P < .07). Conclusions The Lactate Scout has high accuracy and precision, with a negligible bias. It is a reliable POC analyzer to assess l ‐lactate concentration in ovine medicine.