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Evaluation of three different point‐of‐care tests for quantitative measurement of canine C‐reactive protein
Author(s) -
Jasensky AnneKatherine,
Klenner Stefanie,
Einspanier Ralf,
Kohn Barbara
Publication year - 2015
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12264
Subject(s) - point of care testing , medicine , nuclear medicine , pathology
Background C‐reactive protein ( CRP ) is a major acute phase protein in both people and dogs. Objective The objective of this study was to evaluate the precision and accuracy of 3 different point‐of‐care tests ( POCT ) for canine CRP ( cCRP ) in comparison to a standard ELISA test, and to assess storage stability. Material and Methods Blood samples were collected from 125 dogs (23 healthy and 102 diseased). Serum cCRP measurements were performed using the TECO medical AG , the EUROL yser Diagnostica, and the LifeAssays POCT . The TECO medical AG ELISA validated for canine serum was used as a reference method. Statistical analyses included descriptive statistics, inter assay variance for each POCT , comparison of results from each POCT with the ELISA using Spearman's correlation and Bland–Altman plots, and a t ‐test to evaluate sample stability. Results ELISA cCRP values ranged from 0.1–4.7 mg/L (median 1.3) in healthy dogs and from 0–282 mg/L (median 17.3) in diseased dogs. Spearman's correlation coefficient between ELISA and the respective POCT measurements for cCRP concentration was 0.89 for TECO medical, 0.85 for EUROL yser, and 0.97 for LifeAssays. Bias calculated from Bland–Altman difference plots was 27.6% for TECO medical, −14.2% for EUROL yser, and −15.7% for LifeAssays. Inter assay reliability was > 0.9 for all POCT . Total observed error of the EUROL yser was 28.2% and therefore met the acceptable total error of 29.6%. Conclusions All POCT s were able to measure cCRP , but precision, accuracy, and correlation coefficients varied among the 3 systems. Therefore, serial measurements for monitoring of cCRP in dogs should always be performed using the same POCT system.