Premium
Equine bone marrow volume reduction, red blood cell depletion, and mononuclear cell recovery using the PrepaCyte‐CB processing system
Author(s) -
Burton Andrew G.,
Clark Kaitlin C.,
Borjesson Dori L.,
Carrade Danielle D.,
Burges Julie,
Owens Sean D.
Publication year - 2015
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12236
Subject(s) - bone marrow , peripheral blood mononuclear cell , blood volume , medicine , blood cell , red blood cell , andrology , nuclear medicine , pathology , chemistry , immunology , in vitro , biochemistry
Background Volume reduction and RBC depletion of equine bone marrow specimens are necessary processing steps for the immediate therapeutic use of bone marrow ( BM )‐derived mesenchymal stem cells ( MSC ), and for MSC expansion in culture. Objectives The purpose of the study was to evaluate the ability of the PrepaCyte‐ CB processing system to reduce volume, deplete RBC , and recover mononuclear cells ( MNC ) from equine BM specimens. Methods One hundred and twenty mL of heparinized BM were obtained from each of 90 horses. A CBC was performed on the BM pre‐ and post‐PrepaCyte‐ CB processing. Volume and RBC reduction, and total nucleated cell ( TNC ) and MNC recoveries were determined. Results Bone marrow volume was reduced from 120 mL to 21 mL with a median RBC depletion of 90.1% (range, 62.0–96.7%). The median preprocessing total TNC count was 2.2 × 10 9 (range, 0.46–7.9 × 10 9 ) and the median postprocessing TNC count was 1.7 × 10 9 (range, 0.3–4.4 × 10 9 ; P < .0001), with a median recovery of 73.5% (range, 22.4–216.7%). The median preprocessing total MNC count was 0.9 × 10 9 (range, 0.1–4.7 × 10 9 ) and median postprocessing total MNC count was 0.8 × 10 9 (range, 0.1–2.7 × 10 9 ; P = .06), with a median recovery of 83.7% (range, 15.4–413.9%). Conclusions The PrepaCyte‐ CB processing system can be used to deplete both volume and RBC , and recover MNC from equine BM specimens. Further studies assessing the viability of MSC and the efficacy of MSC expansion after using the PrepaCyte‐ CB processing system are warranted.