Premium
Comparison of the platelet‐rich plasma and buffy coat protocols for preparation of canine platelet concentrates
Author(s) -
Hoareau Guillaume L.,
Jandrey Karl E.,
Burges Julie,
Bremer Daphne,
Tablin Fern
Publication year - 2014
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12195
Subject(s) - buffy coat , platelet , chemistry , andrology , in vitro , whole blood , platelet poor plasma , platelet rich plasma , anaerobic exercise , immunology , medicine , biochemistry , physiology
Background Platelet ( PLT ) concentrates ( PC ) can be produced via the buffy coat ( BC ) or platelet‐rich plasma ( PRP ) protocols. The 2 methods have not been compared with canine blood. Objectives The aims of the study were to compare the PLT , WBC , and RBC concentrations, in vitro PLT function, and markers of platelet storage lesion ( PSL ) in canine PC generated by 2 different protocols, and determine microbial growth throughout storage. Methods PC from 8 healthy donor dogs were produced using 2 standard protocols, PRP and BC. PLT, WBC, and RBC counts, optical aggregometry assays, and PSL markers ( pH , p CO 2 , HCO 3 , lactate and glucose concentrations, and LDH activity) were determined on storage days 0, 1, 3, 5, and 7. Aerobic and anaerobic bacterial cultures were also performed. Results Mean PLT counts were comparable between protocols and remained stable throughout storage up to day 7, while median WBC and RBC counts on day 0 were significantly higher in the BC‐PC group (17,800 WBCs/μL; 195,000 RBCs/μL) than in the PRP ‐ PC group (200 WBCs/μL; 10,000 RBCs/μL) ( P = .012). In PRP ‐ PC aggregometry, the median slope and amplitude in response to γ‐thrombin and convulxin (+ ADP ) were significantly decreased, and virtually absent in BC ‐ PC during storage. PSL markers (lactate, LDH activity) were higher in BC ‐ PC . Aerobic bacterial growth was observed in 2 PRP ‐ PC and 1 BC ‐ PC . Conclusions This in vitro study suggests that PRP ‐ PC had lesser WBC and RBC contamination and superior PLT function compared with BC ‐ PC . In vivo studies are required to address safety and efficacy of PRP ‐ PC .