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Relationship between urinary Tamm–Horsfall protein excretion and renal function in dogs with naturally occurring renal disease
Author(s) -
Raila Jens,
Schweigert Florian J.,
Kohn Barbara
Publication year - 2014
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12143
Subject(s) - creatinine , tamm–horsfall protein , renal function , medicine , urinary system , excretion , endocrinology , urine , albuminuria , chemistry , urology
Background Tamm–Horsfall protein ( THP ) is physiologically excreted in urine, but little is known about the role of THP in the diagnosis of renal disease in dogs. Objective The aim of this study was to evaluate to which extent naturally occurring renal disease affects the urinary excretion of THP . Methods Dogs were divided into 5 groups according to plasma creatinine concentration, urinary protein‐to‐creatinine ratio ( UP / UC ), and exogenous plasma creatinine clearance (P‐Cl Cr ) rates: Group A (healthy control dogs; n = 8), nonazotemic and nonproteinuric dogs, with P‐Cl Cr rates > 90 mL/min/m 2 ; group B ( n = 25), nonazotemic and nonproteinuric dogs with reduced P‐Cl Cr rates (51–89 mL/min/m 2 ); group C ( n = 7), nonazotemic but proteinuric dogs with P‐Cl Cr rates 53–98 mL/min/m 2 ; group D ( n = 8), azotemic and borderline proteinuric dogs (P‐Cl Cr rates: 22–45 mL/min/m 2 ); and group E ( n = 15), azotemic and proteinuric dogs (not tested for P‐Cl Cr ). THP was measured by quantitative Western blot analysis, and the ratio of THP ‐to‐urinary creatinine ( THP / UC ) was calculated. Results The THP / UC concentrations were not different among dogs of groups A–D, but were reduced in dogs of group E ( P < .001). THP / UC correlated negatively with serum creatinine ( P < .01) and UP / UC ( P < .01), but was not significantly associated with P‐Cl Cr . Conclusions Decreased levels of THP / UC were present in moderately to severely azotemic and proteinuric dogs. This suggests tubular injury in these dogs and that THP might be useful as urinary marker to study the pathogenesis of renal disease.