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Proportion of mast cells in normal canine hepatic cytologic specimens: comparison of 2 staining methods
Author(s) -
Masserdotti Carlo
Publication year - 2013
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12086
Subject(s) - staining , giemsa stain , pathology , mast cell , cytology , toluidine , stain , systemic mastocytosis , biology , medicine , chemistry , immunology
Background Although the presence of mast cells is considered a normal finding in canine hepatic tissue, the normal proportion of these cells in cytologic specimens from normal livers was not previously established. Objectives The objective of this study was to determine the normal proportion of hepatic mast cells in cytologic specimens from normal canine liver by the use and comparison of the May–Grünwald–Giemsa and the Toluidine Blue staining methods. Methods The cytologic specimens obtained from fine‐needle aspirates of livers of 13 healthy dogs were investigated by counting of hepatocytes and mast cells per high‐power field ( HPF , x40 objective). The ratio of mast cells/100 hepatocytes was then compared between the 2 staining methods. Results A median of 0.17 (range: 0–0.33) mast cells/100 hepatocytes and of 0.47 (range: 0–0.86) mast cells/100 hepatocytes was obtained with May–Grünwald–Giemsa and Toluidine Blue stains, respectively. Conclusions Toluidine Blue stain detects more hepatic mast cells than May–Grunwald–Giemsa in normal canine livers. With both stains, < 1% mast cells were detected in normal canine liver samples.