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Acetylcholinesterase and butyrylcholinesterase activities in obese Beagle dogs before and after weight loss
Author(s) -
Tvarijonaviciute Asta,
Ceron Jose J.,
Tecles Fernando
Publication year - 2013
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12032
Subject(s) - medicine , endocrinology , beagle , butyrylcholinesterase , obesity , pathogenesis , weight loss , cholesterol , high density lipoprotein , population , acetylcholinesterase , chemistry , enzyme , aché , biochemistry , environmental health
Background Obesity is the most common nutritional disorder in today's dog population and the major risk factor for a number of related diseases. However, the exact pathogenesis of obesity‐related complications is not always clear. In people, butyrylcholinesterase ( BC hE) is suspected to be involved in lipoprotein metabolism and has also been associated with the pathogenesis of inflammatory disease, one of the potential complications related to obesity. Objectives The aim of the study was to evaluate the effect of experimentally induced weight loss on BC hE and acetylcholinesterase ( AC hE) in obese dogs to elucidate the possible relationship between these 2 enzymes and obesity. Methods Six obese intact female Beagle dogs were allocated to a weight loss program for 3 months. BC hE was measured in serum samples using butyrylcholine as substrate, whereas AC hE was measured in whole blood after inhibition of BC hE with ethopropazine and using acetylcholine as a substrate. Results After rapid weight loss serum BC hE activities were statistically significantly lower ( P < .05), whereas AC hE activities were higher ( P < .01). There was a positive correlation between serum BC hE activity and concentrations of total cholesterol ( TCHOL , P < .001), low‐density lipoprotein‐cholesterol ( LDL ‐C, P < .001), and triglycerides ( P < .05). A negative correlation was detected between serum BC hE and AC hE activities ( P < .0001), and between AC hE activity and serum levels of TCHOL ( P < .01), LDL ‐C ( P < .01) and high‐density lipoprotein‐cholesterol ( P < .05). Conclusions Short‐term weight loss in obese intact female Beagle dogs resulted in opposite effects in 2 cholinesterase isoenzyme activities, namely lower BC hE and higher AC hE activities.