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Use of real‐time quantitative PCR to document successful treatment of M ycoplasma haemocanis infection with doxycycline in a dog
Author(s) -
Pitorri Francesca,
Dell'Orco Marta,
Carmichael Nick,
Barker Emily N.,
Hollywood Mike,
Tasker Séverine
Publication year - 2012
Publication title -
veterinary clinical pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 51
eISSN - 1939-165X
pISSN - 0275-6382
DOI - 10.1111/vcp.12002
Subject(s) - doxycycline , medicine , hemolytic anemia , real time polymerase chain reaction , anemia , splenectomy , immunology , gastroenterology , spleen , microbiology and biotechnology , biology , antibiotics , gene , biochemistry
An 8‐year‐old J ack R ussell T errier was diagnosed with hemolytic anemia caused by hemoplasmosis 4 years following splenectomy. Quantitative real‐time PCR ( qPCR ) analysis was used initially to confirm infection with M ycoplasma haemocanis and subsequently to monitor and direct medical therapy. Doxycycline was administered beyond resolution of clinical signs until hemoplasma DNA could no longer be detected by qPCR . The dog remained clinically healthy and hemoplasma‐negative 8 months following cessation of therapy. Canine hemoplasmosis should remain as a differential diagnosis for hemolytic anemia in dogs, particularly those that are splenectomized or immunocompromised, even in geographic regions where prevalence of infection is low. Prolonged doxycycline administration has been shown by qPCR to lead to sustained absence of detectable infection and should be considered as a first line treatment for canine hemoplasmosis.