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A newly established canine NK‐type cell line and its cytotoxic properties
Author(s) -
Grudzien Malgorzata,
Pawlak Aleksandra,
Kutkowska Justyna,
Ziolo Ewa,
Wysokińska Edyta,
Hildebrand Wojciech,
ObmińskaMrukowicz Bożena,
Strzadala Leon,
Rapak Andrzej
Publication year - 2021
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12695
Subject(s) - granzyme b , perforin , cytotoxic t cell , granzyme a , granzyme , cd16 , cd8 , natural killer cell , nkg2d , chemistry , microbiology and biotechnology , biology , cd3 , immunology , immune system , biochemistry , in vitro
We established a canine natural killer (NK)‐type cell line called CNK‐89 derived from a dog with NK cell neoplasia. Immunophenotyping analysis showed positive staining for CD5, CD8, CD45, CD56, CD79a and NKp46, while negative for CD3, CD4, CD14, CD20, CD21, CD34, Thy1, IgG, IgM and MHCII. Polymerase chain reaction analysis revealed the presence of CD56, NKG2D, NKp30, NKp44, NKp46 and perforin, but the absence of CD16, Ly49 and granzyme B mRNA. Treating CNK‐89 cells with IL‐2 did not change the expression of activating receptors, TNFα and IFNγ secretion and cytotoxic activity, however, treatment with IL‐12 alone or in combinations with IL‐15, IL‐18 and IL‐21 caused an increase in granzyme B and CD16 mRNA, IFNγ secretion and cytotoxic properties of the CNK‐89 cell line.