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Aberrantly expressed snoRNA, snRNA, piRNA and tRFs in canine melanoma
Author(s) -
Rahman Md. Mahfuzur,
Lai YuChang,
Husna Al Asmaul,
Chen HuiWen,
Tanaka Yuiko,
Kawaguchi Hiroaki,
Hatai Hitoshi,
Miyoshi Noriaki,
Nakagawa Takayuki,
Fukushima Ryuji,
Miura Naoki
Publication year - 2020
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12558
Subject(s) - small nucleolar rna , piwi interacting rna , biology , microrna , small nuclear rna , transfer rna , small rna , rna , downregulation and upregulation , microbiology and biotechnology , melanoma , cancer research , non coding rna , genetics , rna interference , gene
Abstract Among small non‐coding RNAs (sncRNAs/sRNAs), the functional regulation of microRNAs (miRNAs) has been studied in canine oral melanoma (COM). However, the expression level of other sncRNAs, like small nucleolar RNAs (snoRNAs), small nuclear RNAs (snRNAs), transfer RNA‐derived fragments (tRFs) and PIWI‐interacting RNAs (piRNAs), in COM is unknown. The aim of this study was to investigate sncRNAs other than miRNAs in COM from our small RNA sequencing project (PRJNA516252). We found that several snRNAs and piRNAs were upregulated, whereas tRFs and snoRNAs were downregulated in COM. Upregulation of U1 snRNA and piR‐972, and downregulation of tRNA‐ ser (1) and snoRA24 was confirmed in dog melanoma tissue and cell lines by quantitative reverse transcription PCR. Consistently, the expression of tRNA‐ ser (1) and snoRA24 in plasma of COM cases was also decreased. Finally, we found a similar expression trend of U1 and snoRA24 in the human cutaneous melanoma cell line, MEWO, compared with human epidermal melanocyte cells (HEMa‐Lp). In our study, snRNA, snoRNA, tRFs and piRNA were dysregulated during melanoma progression. Moreover, the melanoma‐associated expression of U1 and snoRA24 was similar in human and dog melanoma.