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Feline vaccine‐associated sarcomagenesis: Is there an inflammation‐independent role for aluminium?
Author(s) -
AbdelMageed M. A.,
Foltopoulou P.,
McNiel E. A.
Publication year - 2018
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12358
Subject(s) - chinese hamster ovary cell , aluminium , adjuvant , cytotoxic t cell , inflammation , aluminium chloride , hamster , aluminium hydroxide , dna damage , in vitro , chemistry , cancer research , biology , cell culture , dna , microbiology and biotechnology , immunology , biochemistry , genetics , organic chemistry
Aluminium has been found in feline vaccine‐associated sarcomas. In this study, we investigated the potential for aluminium to contribute directly to tumourigenesis. Our results indicated that an aluminium hydroxide adjuvant preparation was cytotoxic and mutagenic in human‐Chinese hamster ovary ( CHO ) hybrid cells in vitro . Moreover, CHO cells deficient in DNA double strand break ( DSB ), but not single‐strand break ( SSB ), repair, were particularly sensitive to aluminium exposure compared with repair proficient cells, suggesting that aluminium is associated with DSBs . In contrast to CHO cells, primary feline skin fibroblasts were resistant to the cytotoxic effects of aluminium compounds and exposure to an aluminium chloride salt promoted cell growth and cell cycle progression at concentrations much less than those measured in particular feline rabies vaccines. These findings suggest that aluminium exposure may contribute, theoretically, to both initiation and promotion of tumours in the absence of an inflammatory response.