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Transcriptomic profile reveals molecular events associated to focal adhesion and invasion in canine mammary gland tumour cell lines
Author(s) -
Cordeiro Y. G.,
Xavier P. L. P.,
Rochetti A. L.,
Alexandre P. A.,
Mori C. M. C.,
Strefezzi R. F.,
Fukumasu H.
Publication year - 2018
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12339
Subject(s) - biology , mammary gland , transcriptome , extracellular matrix , pathology , cell culture , gene expression , cancer research , cell , cancer , phenotype , in vitro , cell adhesion , in vivo , mammary tumor , cell adhesion molecule , gene , microbiology and biotechnology , medicine , genetics , breast cancer
The prevalence of cancer in animals has increased significantly over the years. Mammary tumours are the most common neoplasia in dogs, in which around 50% are presented in the malignant form. Hence, the development and characterization of in vitro models for the study of canine tumours are important for the improvement of cancer diagnosis and treatment. Thus, the aim of this study was to characterize cell lines derived from canine mammary gland neoplasias which could be further used for basic and applied oncology research. Samples of canine mammary carcinomas were taken for cell culture and 2 cell lines were established and characterized in terms of cell morphology, tumourigenicity and global gene expression. Both cell lines presented spindle‐shape morphology and shown common malignant features as in vitro invasion potential and expression of epithelial and mesenchymal proteins. Also, we found gene expression patterns between the 2 cell cultures in comparison to the normal mammary gland tissue. Cells from M25 culture showed a higher invasion and in vivo tumourigenic potential, associated to the overexpression of genes involved in focal adhesion and extracellular matrix communication, such as FN1 , ITGA8 and THBS2 . The phenotypic characterization of these cells along with their global gene expression profile potentially determine new therapeutic targets for mammary tumours.