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Apoptotic intrinsic pathway proteins predict survival in canine cutaneous mast cell tumours
Author(s) -
Barra C. N.,
Macedo B. M.,
Cadrobbi K. G.,
Pulz L. H.,
Huete G. C.,
Kleeb S. R.,
Xavier J. G.,
CatãoDias J. L.,
Nishiya A. T.,
Fukumasu H.,
Strefezzi R. F.
Publication year - 2018
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12330
Subject(s) - immunohistochemistry , apoptosis , programmed cell death , pathology , mast cell , malignancy , grading (engineering) , caspase , cancer research , cell , radiation therapy , caspase 3 , biology , medicine , immunology , ecology , biochemistry , genetics
Mast cell tumours ( MCTs ) are the most frequent canine round cell neoplasms and show variable biological behaviours with high metastatic and recurrence rates. The disease is treated surgically and wide margins are recommended. Adjuvant chemotherapy and radiotherapy used in this disease cause DNA damage in neoplastic cells, which is aimed to induce apoptotic cell death. Resisting cell death is a hallmark of cancer, which contributes to the development and progression of tumours. The aim of this study was to investigate the expression of the proteins involved in the apoptotic intrinsic pathway and to evaluate their potential use as prognostic markers for canine cutaneous MCTs . Immunohistochemistry for BAX , BCL2 , APAF1 , Caspase‐9, and Caspase‐3 was performed in 50 canine cases of MCTs . High BAX expression was associated with higher mortality rate and shorter survival. BCL2 and APAF1 expressions offered additional prognostic information to the histopathological grading systems. The present results indicate that variations in the expression of apoptotic proteins are related to malignancy of cutaneous MCTs in dogs.