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In vitro and in vivo effect of flutamide on steroid hormone secretion in canine and human inflammatory breast cancer cell lines
Author(s) -
Caceres S.,
Monsalve B.,
Peña L.,
de Andres P. J.,
AlonsoDiez A.,
Illera M. J.,
Woodward W. A.,
Reuben J. M.,
Silvan G.,
Illera J. C.
Publication year - 2018
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12324
Subject(s) - flutamide , in vivo , testosterone (patch) , endocrinology , medicine , dihydrotestosterone , pregnenolone , cell growth , androgen , hormone , androstenedione , in vitro , cell culture , chemistry , steroid , pharmacology , biology , cancer , androgen receptor , prostate cancer , biochemistry , genetics , microbiology and biotechnology
The aim was to study the effects of flutamide on cell proliferation, in vivo tumour growth and steroid production in canine and human IBC cell lines. IPC ‐366 and SUM149 cell cultures were exposed to flutamide concentrations for 72 hours. Additionally, IPC ‐366 and SUM149 xenotransplanted mice were treated subcutaneously with flutamide 3 times a week for 2 weeks. Steroid hormones determination in culture media, serum and tumour homogenates (pregnenolone, progesterone, androstenedione, testosterone, dihydrotestosterone, 17β‐oestradiol and oestrone sulphate) were assayed by EIA . in vitro cell proliferation percentages showed a decrease in all flutamide dosages in IPC ‐366 and SUM149 . in vivo flutamide reduced tumour size by 55% to 65%, and metastasis rates decreased. In treated groups, androgen levels in culture media, serum and tumour homogenates were increased as oestrogen levels decreased. These results suggest that flutamide treatment inhibits cell proliferation and promotes tumour reduction by increasing androgen levels and also support future therapy approaches.