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Comparative analysis of DNA methylation patterns of equine sarcoid and healthy skin samples
Author(s) -
Semik E.,
Ząbek T.,
Gurgul A.,
Fornal A.,
Szmatoła T.,
Pawlina K.,
Wnuk M.,
KlukowskaRötzler J.,
Koch C.,
Mählmann K.,
BugnoPoniewierska M.
Publication year - 2018
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12308
Subject(s) - dna methylation , differentially methylated regions , cpg site , methylation , biology , intergenic region , bisulfite sequencing , illumina methylation assay , methylated dna immunoprecipitation , genomic dna , bisulfite , dna , carcinogenesis , epigenetics , microbiology and biotechnology , genome , gene , genetics , gene expression
Objective In this study, for the first time we report the genome‐wide DNA methylation profile of skin tumour in horses and describe differentially methylated genomic regions (DMRs) with respect to healthy skin. Materials & Methods The comparative analysis of DNA methylation patterns detected using Reduced Representation Bisulfite Sequencing (RRBS) technique, allowed identification of 136 regions showing differential methylation between sarcoid and normal skin tissue. Results Most of the identified DMRs were short fragments, less than 1 kb in size, located in the intergenic regions. Among identified DMRs there were also regions located within genes directly or indirectly related with oncogenesis. We additionally validated 9 CpG sites showing hypomethylation and 9 CpG sites that were hypermethylated in lesional sample, confirming the identified changes in the DNA methylation. Conclusion Knowledge on the changes taking place in the process of DNA methylation may provide a basis for the development of new alternative diagnostic or therapeutic approaches to equine sarcoids.