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Targeting canine mammary tumours via gold nanoparticles functionalized with promising Co( II ) and Zn( II ) compounds
Author(s) -
Raposo L. R.,
RomaRodrigues C.,
Jesus J.,
Martins L. M. D. R. S.,
Pombeiro A. J.,
Baptista P. V.,
Fernandes A. R.
Publication year - 2017
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12298
Subject(s) - doxorubicin , docetaxel , cisplatin , polyethylene glycol , ic50 , chemistry , bovine serum albumin , nanoparticle , pharmacology , paclitaxel , cell culture , viability assay , colloidal gold , cancer , nuclear chemistry , cancer research , chemotherapy , in vitro , nanotechnology , biochemistry , materials science , medicine , biology , genetics
Background Despite continuous efforts, the treatment of canine cancer has still to deliver effective strategies. For example, traditional chemotherapy with doxorubicin and/or docetaxel does not significantly increase survival in dogs with canine mammary tumors (CMTs). Aims Evaluate the efficiency of two metal compounds [Zn(DION) 2 ]Cl (TS262, DION = 1,10‐phenanthroline‐5,6‐dione) and [CoCl(H 2 O)(DION) 2 ][BF 4 ] (TS265) and novel nanovectorizations designed to improve the anti‐cancer efficacy of these compounds in a new CMT derived cell line (FR37‐CMT). Materials and methods FR37‐CMT cells were exposed to different concentrations of TS262 and TS265 and two new nanoparticle systems and cellular viability was determined. These nanosystems are composed of polyethylene‐glycol, bovine‐serum‐albumin and TS262 or TS265 (NanoTS262 or NanoTS265, respectively). Results In FR37‐CMT, TS262 and TS265 displayed IC50 values well below those displayed by doxorubicin and cisplatin. The nanovectorizations further decreased the IC50 values. Discussion TS262 and TS265 proved to be effective against FR37‐CMT cells and more effective than of doxorubicin and cisplatin. The Nanosystems efficiently delivered the cytotoxic cargo inducing a significant reduction of cell viability in FR37‐CMT cell line when compared to the free compounds. Conclusions TS262 and TS265 are compounds with potential in the treatment of CMTs. NanoTS262 and NanoTS265 demonstrate that such simple nanovectorization via gold nanoparticles shows tremendous potential as anti‐cancer formulations, which may easily be expanded to suit other cargo.