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S100A4 (metastasin) positive mesenchymal canine mammary tumour spheroids reduce Tenascin C synthesis under DMSO exposure in vitro
Author(s) -
Kau S.,
Miller I.,
Tichy A.,
Gabriel C.
Publication year - 2017
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12287
Subject(s) - tenascin c , stromal cell , tenascin , in vitro , spheroid , immunohistochemistry , mesenchymal stem cell , chemistry , western blot , cancer research , stimulation , dimethyl sulfoxide , microbiology and biotechnology , cell , biology , immunology , biochemistry , endocrinology , fibronectin , gene , organic chemistry
In breast cancer research S100A4 ‐positive tumour‐associated stromal cells are assumed as primary source of Tenascin C ( TNC ) in the metastatic environment. Aim of the present study was to isolate and characterize S100A4 / TNC positive stromal canine mammary tumour ( CMT ) cells. Cells grown as scaffold‐free spheroids were investigated for S100A4 , TNC , and proliferative activity under 1.8% DMSO stimulation by means of Western blot and immunohistochemistry. DMSO is a commonly used drug solvent despite well‐known side effects on cells including TNC expression. DMSO did not affect proliferation, but TNC was significantly reduced under DMSO exposure for 7 and 14 days, whereby for S100A4 a reducing effect was only observed after 14 days. Without DMSO , cells stably expressed TNC and S100A4 which makes them suitable to be used in experimental approaches requiring S100A4 / TNC expressing CMT stromal cells. Results show that 1.8% DMSO should not be used as solvent for experiments concerning TNC / S100A4 expression.