Premium
Properties of the feline tumour suppressor reduced expression in immortalized cells ( REIC /Dkk‐3)
Author(s) -
Ochiai K.,
Oda H.,
Shono S.,
Kato Y.,
Sugihara S.,
Nakazawa S.,
Azakami D.,
Michishita M.,
Onozawa E.,
Bonkobara M.,
Sako T.,
ShunAi L.,
Ueki H.,
Watanabe M.,
Omi T.
Publication year - 2017
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12254
Subject(s) - immortalised cell line , cell culture , cancer research , apoptosis , clone (java method) , mammary gland , western blot , cell growth , tumor suppressor gene , biology , pathology , cancer , carcinogenesis , medicine , gene , breast cancer , genetics
Reduced expression in immortalized cells ( REIC /Dkk‐3), a member of the human Dickkopf (Dkk) family, is a growth suppressor in human and canine mammary tumours. Mammary gland tumours are common neoplasms with high malignancy in female cats. The purpose of this study was to clone the feline REIC /Dkk‐3 homolog, investigate its expression in cell lines established from feline mammary gland tumours, and test its tumour suppressor function. Western blot analysis revealed that expression of the REIC /Dkk‐3 protein was reduced in feline mammary carcinoma cell lines. Forced expression of REIC /Dkk‐3 induced apoptosis in feline mammary tumour cell lines. These results demonstrate that REIC /Dkk‐3 expression, which is downregulated in feline mammary tumour cell lines, results in the induction of apoptosis in these cells. Our findings suggest that feline REIC /Dkk‐3 represents a potential molecular target for the development of therapies against feline mammary cancers.