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Evaluation of the global DNA methylation in canine mast cell tumour samples by immunostaining of 5‐methyl cytosine
Author(s) -
Morimoto C. Y.,
Tedardi M. V.,
da Fonseca I. I. M.,
Kimura K. C.,
Sanches D. S.,
Epiphanio T. F.,
de Francisco Strefezzi R.,
Dagli M. L. Z.
Publication year - 2017
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12241
Subject(s) - dna methylation , epigenetics , methylation , immunohistochemistry , immunostaining , carcinogenesis , pathology , biology , dna , mast cell , microbiology and biotechnology , cytosine , cancer research , medicine , cancer , immunology , gene expression , genetics , gene
Cutaneous mast cell tumours ( MCT ) are the most common skin tumour in dogs, and to our knowledge, there are no previous studies regarding the global methylation of these tumours. DNA hypomethylation and hypermethylation have been described in several tumours and both mechanisms can lead to carcinogenesis. The purpose of this study was to evaluate the global DNA methylation in canine MCT . A total of 48 MCT samples were classified in grades 1, 2 and 3 or high‐grade or low‐grade. Monoclonal antibodies were used for the immunohistochemical detection of the 5‐methylcytosine. The immunostained nuclei were classified in strong, weak or negative pattern, and these were quantified in five distinct microscopic fields (40× objective) in each slide. The results showed that global DNA hypomethylation was predominant in grade 3, high‐grade, less differentiated MCT . These epigenetic changes in neoplastic mast cells warrant further detailed investigation aiming the establishment of tumour epigenetic therapies.