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Canine cell line, IPC ‐366, as a good model for the study of inflammatory breast cancer
Author(s) -
Caceres S.,
Peña L.,
Lacerda L.,
Illera M. J.,
de Andres P. J.,
Larson R. A.,
Gao H.,
Debeb B. G.,
Woodward W. A.,
Reuben J. M.,
Illera J. C.
Publication year - 2017
Publication title -
veterinary and comparative oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 34
eISSN - 1476-5829
pISSN - 1476-5810
DOI - 10.1111/vco.12238
Subject(s) - flow cytometry , in vivo , mesenchymal stem cell , cell culture , inflammatory breast cancer , cancer , breast cancer , western blot , cancer research , cell , stem cell , medicine , biology , immunology , pathology , microbiology and biotechnology , biochemistry , genetics , gene
Inflammatory breast cancer ( IBC ) is an aggressive type of cancer with poor survival in women. Inflammatory mammary cancer ( IMC ) in dogs is very similar to human IBC and it has been proposed as a good surrogate model for study the human disease. The aim was to determine if IPC ‐366 shared characteristics with the IBC cell line SUM149 . The comparison was conducted in terms of ability to grow (adherent and nonadherent conditions), stem cell markers expression using flow cytometry, protein production using western blot and tumorigenic capacity. Our results revealed that both are capable of forming long‐term mammospheres with a grape‐like morphology. Adherent and nonadherent cultures exhibited fast growth in vivo . Stem cell markers expressions showed that IPC ‐366 and SUM149 in adherent and nonadherent conditions has mesenchymal‐like characteristics, E‐cadherin and N‐cadherin, was higher in adherent than in nonadherent cultures. Therefore, this study determines that both cell lines are similar and IPC ‐366 is a good model for the human and canine disease.